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. 2016 Jan 6;14:5. doi: 10.1186/s12967-015-0761-0

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© Tian et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Human UCB CD34⁺ cells expansion by hD1R requires direct cellular interaction with live HUVECs. a CD34⁺ cells (2 × 10³) were cultured with or without HUVECs in the presence of h5GF plus hD1R. The cells were harvested on day 7 and counted (n = 4). b, c The cells in (a) were analyzed by FACS to determine the percentage (b) and the number (c) of CD34⁺ cells (n = 4). d Representative FACS analysis of CD34⁺ cells in (b). e The cells (1 × 10³) in (a) were used for colony-forming assay (n = 4). f Photomicrographs of human hemopoietic cell colonies. Scale bar represented 200 μm. Bars = mean ± SD. *P < 0.05