Fig. 4.

hD1R enhanced the homing efficiency of CD34⁺ cells. a Homing efficiency. CD34⁺ cells (2 × 10⁵) were purified from UCB (Day 0) or cells expanded ex vivo transplanted into sublethally irradiated mice. BM cells from the recipients were analyzed by FACS 16 h after the transplantation (n = 8). b Migration assay. Freshly purified CD34⁺ cells or cells (2 × 10⁵) expanded ex vivo were cultured in the upper compartment of transwell for 4 h. Total cell migration was quantitated by flow cytometry. Migrated cells toward SDF-1 in the lower chamber significantly increased in hD1R-cultured ones (n = 4). c hD1R increased the expression of integrins in CD34⁺ cells. Human CD34⁺ cells were incubated with HUVECs + h5GF in the presence of PBS or hD1R for 7 days. The hematopoietic cells in suspension or Day 0 CD34⁺ cells were collected, and the expression of α4, α6 integrins, and CXCR4 was estimated by using real time RT-PCR (n = 4). Bars = mean ± SD. *P < 0.05