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Circulating endothelial cell enumeration by flow cytometry. (a) Panels show the gate used to exclude CD45-positive cells; (b) Negative control; (c) Panels show the gate used to count activated circulating endothelial cells (aCECs) (defined as CD45− CD146+ CD105+) pre-therapeutically; (d) Panels show the gate used to count aCECs (defined as CD45- CD146+ CD105+) post-therapeutically.
