Fig. 7.

CXCL5 alone increased neutrophil infiltration and BBB damage and caused white matter injury. Intracerebroventricular infusion of recombinant CXCL5 (2 μg) or NS on P2 revealed that the CXCL5 group (n = 6) had significantly higher ipsilateral ventricle size ratios (a), reduced myelination (MBP) (b), and increased astrogliosis (GFAP) (c) in the white matter on P12 compared with the vehicle group (n = 6). Scale bar = 100 μm. (d) At 24 h after injection, the NS (n = 6) and CXCL5 groups (n = 6) showed no detectable ED1(+) activated microglia in the white matter. By contrast, the CXCL5 group had a significantly higher number of MPO(+) neutrophils and significantly greater BBB damage compared with the vehicle group. Scale bar = 50 μm (ED1) and 100 μm (IgG and MPO); values are means ± SEMs, *p < 0.05, **p < 0.01