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. 2016 Jan 7;49:2. doi: 10.1186/s40659-015-0064-1

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© Beltran et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Competitive exclusion of L. rhamnosus to protect CaCo-2 cell line from V. parahaemolyticus colonization. The figure shows the results about the role of L. rhamnosus strains in the inhibition of V. parahaemolyticus from colonizing colon carcinoma cell line (CaCo-2) monolayers. The strains used were V. parahaemolyticus RIMD 2210633 (RIMD), L. rhamnosus wild type (L. rhamnosus), L. rhamnosus pCWA-MAM-7 (pCWA-MAM7) and L. rhamnosus pSEC-MAM-7 (pSEC-MAM7). Values are expressed as the adhesion percentage of V. parahaemolyticus after 3 h of L. rhamnosus wild type (L. rhamnosus– RIMD), pCWA-MAM-7 (pCWA-MAM7– RIMD) or pSEC-MAM-7 (pSEC-MAM7– RIMD) strains monolayer colonization. V. parahaemolyticus adhesion, without previous Lactobacillus treatment, is expressed as “Adhesion” bar. The figure shows values expressed as the mean ± standard deviation of three full biological replicates, each time in technical triplicate. *p < 0.001 (Student’s-Test)