A schematic of chemical and genetic manipulations of TNF-induced pathways that mediate bacterial killing and macrophage necrosis. Abbreviations: TNFR, TNF receptor; RIP, receptor-interacting protein; MLKL, mixed lineage kinase-like; ROS, reactive oxygen species, CYPD, cyclophilin D; mPTPC, mitochondrial permeability transition pore complex; aSMase, acid sphingomyelinase.