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. 2015 Dec 11;11(12):842. doi: 10.15252/msb.20156308

Figure 5. Selective addiction of EOC to the SIX4 homeobox transcription factor.

Figure 5

  1. Immunoblots indicate miR‐124‐induced SIX4 depletion. siSIX4 is shown as an antibody control.
  2. Consequence of SIX4 depletion on EOC cell viability (as in Fig 2A, N, normal cell lines: HOSE, HBEC30).
  3. In vivo knockdown of SIX4 using neutral liposome‐incorporated siRNA reduced tumor burden and ascites volume in an orthotopic xenograft mouse model. Box‐and‐whisker plot of tumor weight (left panel) or ascites volume (right panel) from n = 7 or 8 mice per condition as indicated. *P‐value from Student's t‐test.
  4. Consequence of SIX4 depletion in PEO1 cells on E‐cadherin plasma membrane accumulation 48 h after transfection. Cells were counterstained with phalloidin and DAPI.
  5. Immunoblots indicate consequence of SIX4 depletion on LKB1 accumulation and AMPK pathway activation.

Source data are available online for this figure.