Figure 6. H₂O₂ increases the frequency of GABAergic mIPSCs .

A, continuous chart recording of GABAergic mIPSCs before during, and after H₂O₂ superfusion. H₂O₂‐induced increases in GABAergic mIPSC frequency were often accompanied by an outward current. B, average frequency (filled circles) and amplitude (open circles) of GABAergic mIPSCs after H₂O₂ superfusion relative to the control as a function of time (mean ± SEM, n = 8). C, summary of GABAergic mIPSC frequency (left) and amplitude (right) relative to the control (mean ± SEM, n = 8 each) showing an increase in frequency but not amplitude induced by H₂O₂. Middle columns of charts indicate maximal values of GABAergic mIPSC frequency and amplitude relative to the control following H₂O₂ superfusion. P values were determined by a paired t test. D, cumulative distributions of inter‐event interval (left) and amplitude (right) of GABAergic mIPSCs before (continuous black line), during (grey line), and after (dotted line) H₂O₂ superfusion. H₂O₂ did not significantly affect the distribution of amplitude (during H₂O₂ superfusion: P = 0.051; after washout: P = 0.061; Kolmogorov–Smirnov test) relative to the control, but shortened the inter‐event interval during H₂O₂ superfusion and increased the interval after washout (P < 0.0001 for each). A and D were obtained from the same neuron. The holding potential was 0 mV for all recordings. Washout indicates the 10 min period after initiating H₂O₂ washout.