Figure 7. H₂O₂‐induced increase in GABAergic mIPSC frequency is due to intracellular Ca²⁺ release via IP₃R .

A, H₂O₂‐induced increase in GABAergic mIPSC frequency was unaffected by Ca²⁺‐free ACSF. B, summary of GABAergic mIPSC frequency relative to the control in Ca²⁺‐free ACSF (mean ± SEM, n = 6). Values in charts correspond to percentage change in maximal values of GABAergic mIPSC frequency relative to the control following H₂O₂ superfusion. P values were determined by a paired t test. C, H₂O₂‐induced increase in GABAergic mIPSC frequency and outward current were completely blocked by 2‐APB (200 μm). D, summary of GABAergic mIPSC frequencies observed during H₂O₂ superfusion with TTX combined with either Stry (2 μm; n = 8), CSA (10 μm; n = 6), Dant (10 μm; n = 6), or 2‐APB (200 μm; n = 7) relative to the control (mean ± SEM). Maximal values of GABAergic mIPSC frequency relative to the control following H₂O₂ superfusion are shown. P values were determined by one‐way ANOVA with a Bonferroni post hoc test. E, FFA (100 μm) did not block the H₂O₂‐induced increase of GABAergic mIPSC frequency. F, summary of GABAergic mIPSC frequency data during FFA treatment (mean ± SEM, n = 6). Values in the charts correspond to the percentage change in maximal values of GABAergic mIPSC frequency relative to the control following H₂O₂ superfusion. The P value was determined by a paired t test. The holding potential was 0 mV for all recordings. Washout indicates the 10 min period after initiating H₂O₂ washout.