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. 2015 Oct 28;593(24):5269–5282. doi: 10.1113/JP271156

Figure 1. IL‐13 impairs ENaC‐dependent Na+ absorption in HT‐29/B6‐GR/MR cells as well as in native intestinal tissue .

Figure 1

A–C, cells were non‐treated (ctrl) or triple‐incubated (DBA, dexamethasone (50 nm), butyrate (2 mm), aldosterone (3 nm)) and treated with or without IL‐13. A, ENaC‐dependent Na+ absorption after 96 h of incubation (J Na) was measured as drop in I SC after addition of amiloride (100 μm). B, time‐dependent progress of J Na. C, IL‐13 dose dependency of J Na. Data in A–C represent means ± SEM; n = 4–10, *P < 0.05, **P < 0.01, ***P < 0.001. D, time course of a representative experiment of DBA‐induced active electrogenic Na+ transport in the distal colon of WT mice that were pretreated with PBS (ctrl) or IL‐13 as described in Methods. Tissues were stimulated with a triple combination of DBA for 8 h and then ENaC‐dependent Na+ absorption (J Na) was determined as drop in amiloride‐sensitive I SC. E, J Na of WT mice treated as described in D given as means ± SEM of 8 mice per group; *P < 0.05, **P < 0.01, ***P < 0.001.