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. 2015 Oct 28;593(24):5269–5282. doi: 10.1113/JP271156

Figure 3. ENaC‐mediated Na+ absorption is suppressed by IL‐13 via inhibition of p38 activity .

Figure 3

A, representative Western blot analysis of both phosphorylated and total ERK1/2, JNK, or p38 proteins after IL‐13 exposition. HT‐29/B6‐GR/MR cells were treated with or without DBA and with or without IL‐13 for 96 h, then rested in media lacking FCS and IL‐13 for 3 h and stimulated again with IL‐13 for 5 min (ERK), 15 min (JNK) or 60 min (p38). Then, total cellular protein was extracted and analysed. B, densitometric measurements were obtained from 3–4 blot membranes representing independent experiments. Data given as means ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001. C, determination of ENaC‐dependent Na+ absorption in the presence or absence of ERK1/2 (U0126) or JNK (SP600125) inhibitors. D, determination of ENaC‐dependent Na+ transport with or without the p38 inhibitor SB202190. Data are given as means ± SEM; n = 6–7, **P < 0.01, ***P < 0.001.