Figure 8. CA12 is required for fluid secretion by salivary glands .

A, cannulation of all the major mouse salivary gland, the procedure used to deliver the adenoviruses. B, ducts were microdissected from the parotid glands of untreated mice (green), mice infected with AdRFP (blue, black) and in addition treated with siCA12 in culture (black), mice infected with AdCA12 (red) and mice infected with AdCA12(E143K). After 48 h in culture, the sealed ducts were used to measure fluid secretion. The results are means ± SEM of the number of ducts (second number) obtained from the number of mice indicated by the first number. C, mice infected with the indicated adenoviruses were used to measure the rate of salivary secretion in response to i.p. injection of 1 mg kg⁻¹ pilocarpine. The collected saliva (C) was used to measure the concentrations of Cl⁻ (D) or Na⁺ (E). The results are means ± SEM of the number of mice indicated in the figure. * denotes p < 0.05 and # denotes p < 0.01 with respect to mice infected with AdRFP.