Fig. 1.
Trans retinoic acid (ATRA) differentiates HL60 acute promyelocytic cell line to neutrophil-like cells. a CD11b and Annexin V staining of HL60 cells treated with various concentrations of ATRA for 3 days. b qPCR of HL60 cells treated with various concentrations of ATRA for 3 days. Data are mean fold-induction of mRNA normalized to untreated HL60 cells (scored as 1) + SD of triplicate wells. c Growth curve of cultured HL60 cells or ATRA-treated HL60 cells over time. Cells were plated at a density of 1 × 105/ml and split when the concentration exceeded 5 × 105/ml. Data are mean cell counts +/− SD of triplicate culture plates (d) 4,6-diamidino-2-phenylindole staining of nuclear morphology of ATRA-treated or untreated HL60 cells. All data are representative of three independent experiments. PAD peptidylarginine deiminase