Fig. 2.

Ionomycin, phorbol 12-myristate 13-acetate (PMA) and UV-B induce distinct forms of granulocyte cell death. a Trans retinoic acid (ATRA)-differentiated HL60 cells treated with no stimulation, Ionomycin, PMA, or UV-B and stained with 4,6-diamidino-2-phenylindole. b Unstimulated (NO STIM) and Ionomycin-treated (IONO) granulocytes screened by western blot for citrullination of H3. Blots were stripped and reprobed for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a load control. c Flow cytometry (ungated) of ATRA-differentiated HL60 cells treated with Ionomycin, PMA or UV-B and stained with Annexin V and cell impermeant nucleic acid stain (TOPRO). Data are representative of at least three independent experiments