Figure 5.

AtPAT14 is involved in early senescence. A, Chlorophyll content analysis. Left, The first pair of true leaves from 28-d plants were used to measure the chlorophyll content. B, Transcript level of SAG12. C, Transcript level of SAG101. D, Transcript level of the pathogen related gene 1, PR1. Seven-day-old plate-grown seedlings and the sixth and seventh rosette leaves of 28-d- and 35-d-old soil-grown plants were collected and used for RNA extraction followed by RT and real-time PCR. The gene expression level in the seedlings of 7-d-old wild type (WT) is regarded as 1, and the relative expression level of all four genes was calculated. *P < 0.05 in Student’s t test in A, and the different letters above the columns in B to D indicate statistically different values analyzed by one-way ANOVA.