Table II. List of the top 25 genes based on degree of connection in each of two network approaches, NetworkAnalyzer (Cytoscape) and WGCNA (FA centered).
Sixteen genes are in common between the two lists, resulting in a total of 35 genes over the two approaches. √ indicates a significant association of a gene with FAs in WGCNA. The dashes indicate that there is no significant association of a gene with the specific FA in WGCNA. For the MapMan pathway, 1 = exotics, 2 = FA synthesis and FA elongation, 3 = glycerol metabolism, 4 = lipid degradation, 5 = lipid signaling, 6 = lipid transfer proteins, and 7 = phospholipid synthesis.
| bhhhhhhhh | Gene Symbol | BraID | Network Analysis Method | Degree of Connection |
eQTL |
SFAs |
MUFAs |
PUFAs |
MapMan Pathway | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NetworkAnalyzer | WGCNA | Cis- | Trans- | Myristic | Stearic | Palmitic | Arachidic | Behenic | Palmitoleic | Oleic | Eicosenoic | Erucic | Docosadienoic | Linoleic | Eicosadienoic | |||||
| 1 | PLA2-ALPHA | Bra038125 | Both | 91 | 5 | A05 | – | √ | – | – | – | – | √ | – | – | – | √ | √ | √ | 4 |
| 2 | Alcohol oxidase | Bra013391 | Both | 90 | 2 | – | A09 | √ | – | – | – | – | √ | – | – | – | – | – | – | 4 |
| 3 | Choline monooxygenase | Bra024118 | Both | 88 | 3 | – | A02 | √ | √ | – | – | – | √ | – | – | – | – | – | – | 7 |
| 4 | Lysophospholipases | Bra003814 | Both | 87 | 4 | – | A09 | – | √ | √ | – | – | √ | – | – | √ | – | – | – | 4 |
| 5 | Choline kinase | Bra028032 | Both | 87 | 4 | A09 | A03, A08 | √ | – | √ | – | – | √ | – | – | √ | – | – | – | 7 |
| 6 | Hydrolase | Bra016558 | Both | 87 | 2 | – | A03, A09 | √ | – | – | – | – | √ | – | – | – | – | – | – | 4 |
| 7 | Alcohol oxidase | Bra012548 | Both | 87 | 2 | A03 | A09 | – | – | √ | – | – | √ | – | – | – | – | – | – | 4 |
| 8 | LPAT3 | Bra030448 | Both | 84 | 6 | A05 | A09 | √ | √ | √ | – | – | √ | – | √ | √ | – | – | – | 7 |
| 9 | CER10 | Bra007154 | Both | 81 | 3 | A09 | A01 | – | – | √ | – | – | √ | – | – | √ | – | – | – | 1 |
| 10 | IBR10 | Bra039860 | Both | 79 | 2 | A08 | A09 | √ | – | – | – | – | √ | – | – | – | – | – | – | 4 |
| 11 | NPC4 | Bra021355 | Both | 78 | 4 | – | A03, A09 | √ | – | √ | – | – | √ | – | – | √ | – | – | – | 4 |
| 12 | Stearoyl-ACP desaturase | Bra021427 | Both | 78 | 2 | – | A09 | √ | – | – | – | – | √ | – | – | – | – | – | – | 2 |
| 13 | Lipases | Bra035263 | Both | 77 | 3 | – | A03, A07, A09 | – | – | √ | – | – | √ | – | – | √ | – | – | – | 4 |
| 14 | AT3BETAHSD | Bra015621 | Both | 74 | 2 | A10 | A05 | – | – | √ | – | – | √ | – | – | – | – | – | – | 1 |
| 15 | GPDHC1 | Bra029669 | Both | 73 | 2 | A05 | A07, A09 | √ | – | – | – | – | √ | – | – | – | – | – | – | 3 |
| 16 | SDP6 | Bra035180 | Both | 69 | 3 | A07 | A05, A08, A09 | √ | – | √ | – | – | √ | – | – | – | – | – | – | 3 |
| 17 | MOD1 | Bra013159 | NetworkAnalyzer | 86 | 1 | A03 | A04, A05, A09 | – | – | – | – | – | √ | – | – | – | – | – | – | 2 |
| 18 | Triacylglycerol lipase | Bra007686 | NetworkAnalyzer | 86 | 1 | A09 | – | – | – | – | – | – | √ | – | – | – | – | – | – | 4 |
| 19 | CAC3 | Bra000037 | NetworkAnalyzer | 85 | 1 | A03 | – | – | – | – | – | – | √ | – | – | – | – | – | – | 2 |
| 20 | ACP dehydratase | Bra038539 | NetworkAnalyzer | 84 | 1 | A09 | A02 | – | – | – | – | – | √ | – | – | – | – | – | – | 2 |
| 21 | Stearoyl-ACP desaturase | Bra008631 | NetworkAnalyzer | 77 | 1 | – | A09 | – | – | – | – | – | √ | – | – | – | – | – | – | 2 |
| 22 | ACP1 | Bra039471 | NetworkAnalyzer | 77 | 1 | – | A09 | – | – | – | – | – | √ | – | – | – | – | – | – | 2 |
| 23 | ACBP4 | Bra039439 | NetworkAnalyzer | 75 | 1 | A05 | A01, A09 | – | – | – | – | – | √ | – | – | – | – | – | – | 2 |
| 24 | Lipases | Bra002174 | NetworkAnalyzer | 73 | 1 | – | A04 | – | – | – | – | – | √ | – | – | – | – | – | – | 4 |
| 25 | LTP5 | Bra038908 | NetworkAnalyzer | 71 | 1 | A01 | A06, A09 | – | – | – | – | – | √ | – | – | – | – | – | – | 6 |
| 26 | MD-2 | Bra001421 | WGCNA | 69 | 3 | A03 | A05, A09 | – | – | – | – | – | – | √ | – | √ | – | √ | – | 8 |
| 27 | WD-40 | Bra001726 | WGCNA | 62 | 5 | A03 | A05 | – | √ | – | √ | – | – | √ | √ | √ | – | – | – | 4 |
| 28 | LTP4 | Bra020323 | WGCNA | 52 | 3 | – | A06, A08, A09 | – | √ | – | – | – | – | – | √ | √ | – | – | – | 6 |
| 29 | ACBP3 | Bra019240 | WGCNA | 51 | 3 | A03 | A05, A07 | – | √ | – | – | – | – | – | √ | √ | – | – | – | 2 |
| 30 | LTP5 | Bra012847 | WGCNA | 31 | 3 | A03 | – | – | √ | – | – | – | – | – | √ | √ | – | – | – | 6 |
| 31 | Protein kinase | Bra034040 | WGCNA | 13 | 3 | – | A04, A10 | – | – | √ | – | √ | – | – | √ | – | – | – | – | 4 |
| 32 | mtACP2 | Bra035355 | WGCNA | – | 3 | – | A04 | – | – | √ | – | – | – | – | √ | √ | – | – | – | 2 |
| 33 | phosphoethanolamine NMT2 | Bra018740 | WGCNA | – | 5 | No eQTL | No eQTL | – | √ | √ | – | – | √ | – | √ | √ | – | – | – | 7 |
| 34 | Unknown | Bra001486 | WGCNA | – | 3 | No eQTL | No eQTL | – | – | √ | – | – | – | – | √ | √ | – | – | – | 5 |
| 35 | ATVPS34 | Bra027152 | WGCNA | – | 3 | No eQTL | No eQTL | – | √ | – | – | – | – | – | √ | √ | – | – | – | 5 |