Figure 3.

Analysis of BHTC activity in Arabidopsis Col-0 or Col-0 defense mutants. Experiments were conducted with 3-week-old soil-grown seedlings sprayed with 100 µm BHTC, 100 µm DCA, or mock solution (1% [v/v] DMSO) 24 h prior to infection with 3 × 10⁴ virulent Hpa Noco2 spores mL⁻¹ (2 mL per pot). Spores were counted at 7 dpi. Shown are relative numbers of spores per seedling compared with values obtained with the respective mock-treated controls. Error bars represent se based on at least four independent biological replicates. For each replicate, at least three pots, each containing more than 20 seedlings, were examined. Relative spore numbers that are significantly reduced based on Student’s t test (P < 0.05) are marked by asterisks. w, wrky.