Figure 3.
Identification of PHL2 and PHL3 as the P sequence-binding proteins by Y1H assay. A, Yeast cells harboring the 3×P:AbAr reporter gene were transformed with the expression vector carrying a fusion gene of the GAL4 activation domain and the CDS of PHL2 or PHL3. The transformants were grown on control (SD/-Leu) and selection medium (SD/-Leu/200 nm aureobasidin A). B, The relative position of PHL2 and PHL3 in the MYB-CC family. The diagram showing the phylogenetic relationship of MYB-CC proteins is modified from Bustos et al. (2010). C, Alignment of protein sequences of PHL2, PHL3, PHR1, and PHL1. The two conserved regions corresponding to the MYB domain and coiled-coiled domain are indicated in red and blue boxes, respectively.