Figure 2.

Yeast two-hybrid assays between PARC6 stromal regions and the stromal chloroplast division proteins FtsZ1, FtsZ2, and ARC3. Growth of Y2HGold cells was assayed in the presence (His) or absence (−His) of His to detect the activation of the HIS3 reporter. The latter medium was supplemented with 0.1 μg mL⁻¹ of the toxic drug aureobasidin A (AbA) to detect two-hybrid interactions based on activation of the AUR1-C reporter, which confers aureobasidin A resistance in the Y2HGold strain. Constructs were expressed in the pGAD-T7 (AD) or pGBK-T7 (BD) vector as indicated. Empty vectors were used as controls. Dilutions from the same starting culture are indicated at bottom. Predicted transit peptides were excluded from all constructs. Construct and strain details are described in “Materials and Methods.”