Figure 1.

Rescue sim-1 smr1-1-conferred phenotypes by a SMR1 genomic fragment. A, Scanning electron micrographs. Two-week-old plants were observed with a scanning electron microscope. Bars in Col-0, sim-1, and smr1-1 are 100 μm. Bars in sim-1 smr1-1 and the lines 5 and 7 are 50 μm. B, The SMR1 construct used for the rescue experiment. The position of the smr1-1 mutation was indicated. C, Expression of SMR1 in transgenic sim-1 smr1-1 plants. A construct containing a 2367 bp SMR1 genomic fragment was used to transform the sim-1 smr1-1 mutant, and independently transformed homozygous lines (4, 5, 7, 9, 15, 16, and 17) were obtained. Total RNA was extracted and analyzed by northern blotting. These experiments were repeated twice with similar results.