Figure 2.

SMR1 plays a stronger role than SIM in regulating ploidy of leaf cells. A, Analysis of leaf cell ploidy by flow cytometry. The fourth to sixth leaves of 25-d-old plants were collected for nuclei isolation, followed by flow cytometry analysis. Data represent the average of five experiments ± se of mean. Statistical analysis was performed with Mann-Whitney test (http://vassarstats.net/). Asterisks indicate significant difference between samples of different genotypes in the same ploidy group (P < 0.05). Ploidy indices are shown above the bars. B, Epidermal cell morphology. The fourth to sixth leaves of 25-d-old plants were cleared overnight with ethanol and photographed using a dissecting microscope connected to a CCD camera. Highlighted shapes indicate typical pavement cells in each genotype. Note giant pavement cells in Col-0 and sim-1, but not in smr1-1 and sim-1 smr1-1. Bars, 100 μm.