Figure 3.

Correlation between peroxisome displacement during the recovery period and peroxule tip displacement during translation indicates anchored tethering between chloroplasts and peroxisomes. Peroxule tip displacement during the translation period was plotted against the peroxisome displacement during the recovery period for cp (A; n = 37) and noncp (B; n = 46) peroxisomes. Peroxisomes were trapped with 37-mW optical trap laser power followed by a 21.5-s recovery period. The behavior of cp samples is indicative of anchored tethers, where the peroxule tip represents the base of the tether: small peroxule tip displacement combined with large peroxisome recovery displacement (circle). Note that the sample sizes are different from those in Supplemental Table S1, as displacement could only be measured if the peroxule was observable for the entire period.