Figure 4.

Compound effects on endosomal trafficking. A and B, Confocal images of PIN2-GFP-expressing root epidermal cells with indicated treatments. A, BFA (50 μm) supplementation causes the formation of endosomal aggregates or BFA compartments (arrow). PIN2-GFP-expressing roots were pretreated with compound for 30 min prior to the addition of BFA for an additional hour. B, PATI-1 and -2 retain BFA bodies in washout experiments. Roots were treated with BFA for 2 h, followed by 2 h of washouts in the presence of compound. BFA w/o, BFA washout. C, Compounds do not inhibit BFA-induced VHA-a1-GFP endosomal aggregate formation. Confocal images of VHA-a1-GFP-expressing root epidermal cells with indicated treatments. Bar, 25 µm.