Figure 8.

FIT protein abundance in zat12-3 and in response to H₂O₂. Anti-HA immunoblot analysis of HA-FIT protein abundance in HA-FIT/WT and HA-FIT/zat12-3 seedlings grown for 10 d on sufficient (+Fe; A and B) and deficient (−Fe; C and D) Fe supply is shown. Plants were incubated with 20 mm H₂O₂ (+) or water (−) for 1 h prior to harvesting for analysis. Arrowheads indicate the positions of the bands corresponding to HA-FIT (55 kD). Wild-type (WT) seedlings were used as a negative control. Ponceau S staining of the membrane was used as a loading control. B and D show quantification of A and C, respectively, based on three independent experiments. Bars represent normalized protein abundance. Error bars represent sd. Different letters indicate statistically significant differences (P < 0.05).