Figure 4.

Effect of exogenous Put on Fe deficiency symptoms in Arabidopsis wild type and noa1 and nia1nia2 mutants. Six-week-old seedlings were treated with or without 0.1 mm Put in the presence or absence of 50 μm Fe for 7 d, the Fe deficiency symptoms (A) were taken using a digital camera, and root and shoot samples were taken to measure the root soluble Fe content (B), shoot soluble Fe content (C), root FCR activity (D), the expression of genes related to Fe uptake (E, F, G), cell wall Fe content (H), cell wall hemicellulose content (I), and Fe content in the hemicellulose (J). Expression levels in the +Fe-treated sample for each gene were set to 100%, and levels in the tested sample were calculated relative to the corresponding control value. Error bars represent ± sd (n = 4). Different letters represent significant difference at P < 0.05.