Table 2. Phylogenetic clusters of the thermophilic isolates and reference strains derived from their 16S rRNA genes, BOX- and (GTG)5 elements and also ITS regions.
| Bacteria | Phylogenetic groups | |||||
|---|---|---|---|---|---|---|
| 16S rRNA gene identities | 16S rRNA gene | ITS region | (GTG)5 element | BOX element | Cumulative clusters | |
| Genus Geobacillus | ||||||
| G. vulcanii DSM 13174T | 99.0-99.8% to DSM 13174T, DSM 5366T and DSM 7263T | G-2 | ITS-1 | GTG-1 | BOX-1 | 5 |
| Geobacillus sp. E173b | G-1 | ITS-2 | GTG-1 | BOX-1 | 5 | |
| Geobacillus sp. D413 | G-1 | ITS-3 | GTG-2 | BOX-2 | 5 | |
| Geobacillus sp. C304 | G-1 | ITS-4 | GTG-2 | BOX-2 | 5 | |
| Geobacillus sp. A353 | G-1 | ITS-5 | GTG-3 | BOX-3 | 15 | |
| G. thermoleovorans DSM 5366T | G-3 | ITS-6 | GTG-1 | BOX-4 | 5 | |
| G. kaustophilus DSM 7263T | G-4 | ITS-7 | GTG-4 | BOX-5 | 5 | |
| G. stearothermophilus A113 | 99.8% to DSM 22T | G-5 | ITS-8 | GTG-5 | BOX-6 | 2 |
| G. stearothermophilus ATCC 43223T | G-5 | ITS-8 | GTG-5 | BOX-6 | 2 | |
| G. stearothermophilus ATCC 12980T | G-5 | ITS-8 | GTG-6 | BOX-7 | 2 | |
| G. stearothermophilus ATCC 7953 | G-5 | ITS-8 | GTG-6 | BOX-7 | 2 | |
| G. thermodenitrificans D195 | 98.7-99.2% to DSM 465T | G-6 | ITS-9 | GTG-7 | BOX-8 | 14 |
| G. thermodenitrificans A333 | G-6 | ITS-10 | GTG-7 | BOX-8 | 14 | |
| G. thermodenitrificans DSM 465T | G-6 | ITS-11 | GTG-7 | BOX-8 | 14 | |
| G . themodenitrificans subsp. callidus F84a | 98.3% to DSM 22629T | G-7 | ITS-12 | GTG-8 | BOX-9 | 14 |
| G . themodenitrificans subsp. callidus F84b DSM 22629T | G-7 | ITS-12 | GTG-8 | BOX-9 | 14 | |
| G. thermoglucosidasius B84a | 99.2% to DSM 2542T | G-8 | ITS-13 | GTG-9 | BOX-10 | 11 |
| G. thermoglucosidasius DSM 2542T | G-8 | ITS-14 | GTG-10 | BOX-11 | 11 | |
| G. toebii E134 | 99.2% to DSM 14590T | G-9 | ITS-15 | GTG-11 | BOX-12 | 9 |
| G. toebii DSM 14590T | G-9 | ITS-16 | GTG-11 | BOX-12 | 9 | |
| Genus Thermolongibacillus | ||||||
| T. kozakliensis E173a | 97.5% to each other, 94.2-95.0% to DSM 14590T | T-1 | ITS-17 | GTG-12 | BOX-13 | 17 |
| T. altinsuensis E265 | T-2 | ITS-17 | GTG-13 | BOX-14 | 4 | |
| Genus Aeribacillus | ||||||
| Aeribacillus sp. A364 | 98.0-99.2% to DSM 3670T | Ae-1 | ITS-18 | GTG-14 | BOX-15 | 18 |
| Aeribacillus sp. C196 | Ae-1 | ITS-18 | GTG-15 | BOX-15 | 8 | |
| Aeribacillus sp. D642 | Ae-1 | ITS-18 | GTG-16 | BOX-15 | 13 | |
| Aeribacillus sp. E334 | Ae-1 | ITS-18 | GTG-16 | BOX-15 | 13 | |
| A. pallidus DSM 3670T | Ae-1 | ITS-18 | GTG-16 | BOX-15 | 10 | |
| Genus Anoxybacillus | ||||||
| A. ayderensis NCIMB 13972T | 99.7-94.3% to each other | An-1 | ITS-19 | GTG-17 | BOX-16 | 7 |
| A. flavithermus DSM 2641T | An-2 | ITS-20 | GTG-18 | BOX-17 | 3 | |
| A. kamchatkensis DSM 14988T | An-3 | ITS-21 | GTG-19 | BOX-18 | 1 | |
| A. amylolyticus DSM 15939T | An-4 | ITS-22 | GTG-20 | BOX-19 | 7 | |
| A. rupiensis DSM 17127T | An-5 | ITS-23 | GTG-21 | BOX-20 | 19 | |
| A. voinovskiensis DSM 17075T | An-6 | ITS-24 | GTG-22 | BOX-21 | 16 | |
| A. thermarum DSM 17141T | An-7 | ITS-25 | GTG-23 | BOX-22 | 1 | |
| A. calidus C161ab | 96.8% to DSM 17127T and DSM 17075T | An-8 | ITS-26 | GTG-24 | BOX-23 | 12 |
| A. caldiproteolyticus A413 | 97.9-99.9% to DSM 15730T | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 |
| A. caldiproteolyticus A404 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus D504 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus DSM 15730T | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus A403 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus A394 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus A412b | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus C226 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus D621 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus D623 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus A146 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus A142 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus D494 | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus A392b | An-9 | ITS-5 | GTG-25 | BOX-3 | 6 | |
| A. caldiproteolyticus A335 | An-9 | ITS-5 | GTG-26 | BOX-3 | 6 | |
| A. tepidamans DSM 16325T | 96.0-98.1% to DSM 16325T | An-10 | ITS-27 | GTG-27 | BOX-24 | 19 |
| Genus Bacillus | ||||||
| B. licheniformis DSM 13T | – | B-1 | ITS-28 | GTG-28 | BOX-25 | 20 |
1
Abbreviations: The unique and distinctive fingerprinting profiles (% similarities) were written in bold character. The cumulative clusters obtained from three of these analyses were indicated in the right column. 16S rRNA gene groups: G-; Geobacillus, Ae-; Aeribacillus, An-; Anoxybacillus, B-; Bacillus, T-; Thermolongibacillus, BOX-; groups for BOX element, GTG-; groups for (GTG)5 elemet, ITS-; groups for ITS region.