Figure 3. Detection of 16S rRNA gene (panels a, c, d) and phylogenetic tree based on 16S rRNA gene sequences (panel b) of R. erythropolis IBBPo1 cells after 1% organic solvents exposure. Panel a. 16S rRNA gene; Panel c. 16S rRNA gene digested with restriction endonuclease EcoRI; Panel d. 16S rRNA gene digested with restriction endonuclease XbaI; bacterial cells cultivated 1 h (lanes 1, 3, 5, 7, 9, 11, 13) and 24 h (lanes 2, 4, 6, 8, 10, 12, 14) in minimal medium; control (lanes 1, 2), cyclohexane (lanes 3, 4), n-hexane (lanes 5, 6), n-decane (lanes 7, 8), toluene (lanes 9, 10), styrene (lanes 11, 12), ethylbenzene (lanes 13, 14); undigested 16S rRNA gene (lane 15); 1 kb DNA ladder (lane M). Panel b. The phylogenetic tree was obtained using the neighbor-joining method in MEGA5.1 program. The scale bar indicates substitutions per nucleotide position.
