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Secretion of secondary metabolites by the A. niger laeA mutant on minimal medium (MM) agar plates and MM-shake flask cultures. Spores of the wild-type and mutant were streaked to single colonies on MM agar plates and incubated at 30° for 5 d. For batch cultures, spores were inoculated at a density of 1 × 106 spores/ml and grown at 30° for 5 d.
