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. 2016 Jan 7;11(1):e0146410. doi: 10.1371/journal.pone.0146410

Fig 2. The permissiveness of mice BMDMs to legS2 mutant bacteria is independent of caspase-1 or caspase-7 activation.

Fig 2

(A) Caspase-1 KO (casp-1-/-) macrophages were infected with L. pneumophila, JR32, or legS2 with an MOI of 0.5 for 1, 24, 48 and 72 h; then, CFUs were measured at the indicated time points. (B) Levels of IL-1β were detected in supernatants of WT or casp-1-/- BMDMs were infected with JR32 or the legS2 mutant after 24 hr, while WT BMDMs were either not treated (NT) or infected with L. pneumophila, JR32, or legS2 mutant bacteria for 2 h. Salmonella infection (Sal) was used as a positive control for caspase-1 or caspase-7 activation. (C) Casp-1 or (D) casp-7 antibodies were used to detect casp-1 and casp-7 activation, respectively, in cell extracts.