Fig 5. Increased replication of the legS2 mutant in BMDMs is associated with reduced cytokine production.

WT BMDMs or casp-1^-/- macrophages were infected with L. pneumophila, JR32, or the legS2 mutant. 24 h post infection, the supernatants from infected and un-infected cells were assayed by ELISA. (A) IL-10 level, (B) IL-6, (C) TNFα levels. Data represent the mean + SD of n = 3. Asterisks indicate significant differences (***P<0.001), and a two tailed t-test was used to calculate the P-value.