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. 2015 Dec 30;21:1357–1367.

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Copyright © 2015 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Wound healing rate is decreased in diabetic limbal epithelial cells compared to healthy ones as revealed by scratch wound assay in the presence of 1 and 4 ng/ml epidermal growth factor. A: Wound healing in the presence of 1 and 4 ng/ml EGF was evaluated by measuring wound areas for healthy (NL) and diabetic (DM) LEC using ImageJ software and presented as percentage of wound area for healthy (NL) and diabetic (DM) LEC using ImageJ software and presented as percentage of wound area coverage ± standard error of mean. * p<0.05; *** p<0.0001 versus healthy controls. B: Representative images of healthy and diabetic limbal epithelial cells (LECs) after 20 h of wound healing in the medium with 1 ng/ml epidermal growth factor (EGF). Vertical lines demarcate the borders of the original wound. Bar=500 µm.