Table 2. List of primers.
| mRNA | Up primer 5′ to 3′ | Dp primer 5′ to 3′ | Amplicon size (bp) |
|---|---|---|---|
|
Perlecan |
CGTGGCAGTCAACACCAAAG |
TTCTTGATGCAGCCCGTGAT |
105 |
|
Nidogen-1 |
ATGGTTCAGCATCACTGGG |
TAATGACCAGCTTGCCTGGG |
80 |
|
Nidogen-2 |
CGTGCAAGACACGGAAGTCA |
GAGTTGGCTGGGACGTAAGG |
141 |
|
Lumican |
TGCAGCTTACCCACAACAAG |
TGAAGGTGAACGAAGGTCAA |
76 |
|
Keratocan |
AGTGCGGATGACTTTGATTG |
TGGCTTCTCTGGAATGGTTT |
165 |
|
α-SMA |
ATTGTGCTATGTCGCTCTGG |
GATGAAGGAGGGCTGGAA |
157 |
| β-actin | CTGGAACGGTGAAGGTGACA | CGGCCACATTGCAGAACTTT | 73 |