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. 2016 Jan 7;11(1):e0146482. doi: 10.1371/journal.pone.0146482

Fig 1. MIF secretion is induced by IR.

Fig 1

A) Western blot of RCC4 cells treated with 4 Gy IR and lysed at various time points after exposure stained with phosphoserine 15 p53, MIF, and β-actin antibodies. MIF levels were found to decrease within 0.25 hours and recover by 24 hours. B) Western blot of 786-O cells treated with 4 Gy IR and lysed at various time points after exposure. C) Western blot of MCF7 cells treated with 4 Gy IR and lysed at various time points after exposure. D-F) MIF ELISA analysis for cells irradiated in panels (A-C) at the one hour time point.