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A) Immunohistochemical MIF staining on 786-O tumor sections at 0.5, 2, 6, or 24 hours after 4 Gy IR. Different tumors were used for each time point. Scale bar = 50 μm B) ELISA of circulating human MIF levels in mouse sera from 786-O tumor bearing mice at 0.5, 2, 6, or 24 hours after 4 Gy IR. The numbers of animals for each point are indicated. Statistical significance measured by one-way ANOVA.
