ICA69-specific autoreactive T-cells derived from Ica1 deficient thymi are sufficient to transfer multi-organ autoimmunity. A. Athymic nude mice transplanted with Ica1-deficient thymi were sacrificed at 16–24 weeks. Representative cryosections of the pancreas (PANC), the salivary glands (SG) and the stomach (STOM) were stained with anti-CD4 (red) and anti-insulin (green, for the pancreatic sections only) antibodies. No infiltrate was observed in control nude transplanted with wild type thymi (not shown). B. T-cells harvested from the Aire-ΔICA69 mice were adoptively transferred to immunocompromised Rag1−/− mice. Representative cryosections of the salivary glands (SG), parotid glands (PG), the thyroid (ThyG) and the tear glands (TG) were stained with anti-CD4 antibodies (red), and counter-stained with Hoechst 33342 (blue) for nucleus.