. 2016 Jan 7;11(1):e0146571. doi: 10.1371/journal.pone.0146571

Table 1. Behaviour in LD/DD conditions.

Group	Genotype	n	period	sem	Rhythmic %
Over expression of sgg	P{EP}sggEP1576 w¹¹¹⁸; Pdf-Gal80/+; cry-Gal4/+	32	23,70	0,08	97
	P{EP}sggEP1576 w¹¹¹⁸; cry-Gal4/+	27	24,91	0,18	91
	P{EP}sggEP1576 w¹¹¹⁸; tim-Gal4/+	29	21,37	0,36	76
	P{EP}sggEP1576 w¹¹¹⁸;; Clk 4-1M Gal4/+	16	24,38	0,06	100
	P{EP}sggEP1576 w¹¹¹⁸;tim-Gal4,pdf-Gal80/+	14	24,83	0,13	89
Over expression of sgg (Uas)	y w; Pdf- Gal80/+; P{UAS-sgg.B}MB5/cry-Gal4	22	23,72	0,14	83
	cry-Gal4/+;P{UAS-sgg.B}MB5/+	29	20,94	0,14	77
	tim-Gal4/+; P{UAS-sgg.B}MB5/+	21	18,55	0,62	61
	P{UAS-sgg.B}MB5/ Clk 4-1M Gal4	16	24,16	0,07	100
Down regulation of sgg (RNAi)	Pdf-Gal80/+; P{TRiP.GL00277 sgg RNAi}attP2/cry-Gal4	29	24,20	0,14	93
	cry-Gal4/+;P{TRiP.GL00277 sgg RNAi}attP2/+	28	26,21	0,15	92
	tim-Gal4/+; P{TRiP.GL00277 sgg RNAi}attP2/+	31	27,03	0,14	100
	P{TRiP.GL00277 sgg RNAi}attP2/Clk 4-1M Gal4	16	24,36	0,23	100
	P{TRiP.GL00277 sgg RNAi}attP2;35364xtim-Gal4,pdf- Gal80/+	16	25,29	0,13	56
Over expression of per	w;pdfGal80; UAS-per16/cry-Gal4	13	24,18	0,04	77
	w;cry-Gal4/+;UAS-per16/+	14	23,90	0,07	57
	w;tim-Gal4/+; UAS-per16/+	13	26,07	0,10	100
	w;;UAS-per16/ Clk 4-1M Gal4	13	24,64	0,10	92
	w;tim-Gal4,pdf-Gal80/+;UAS-per16/+	14	24,76	0,10	57
Control animals	w¹¹¹⁸; pdf- Gal80/+;cry-Gal4/+	29	23,66	0,12	97
	w¹¹¹⁸; cry-Gal4/+	28	24,69	0,17	92
	w¹¹¹⁸; tim-Gal4/+	29	23,84	0,11	97
	w¹¹¹⁸;; Clk 4-1M Gal4/+	32	23,90	0,11	97
	w¹¹¹⁸; tim-Gal4,pdf- Gal80/+	14	23,61	0,17	71
	P{EP}sggEP1576, w¹¹¹⁸	27	24,09	0,11	87
	w¹¹¹⁸;;UAS-per16/+	13	23,63	0,08	92
	w¹¹¹⁸;;P{UAS-sgg.B}MB5/+	30	23,47	0,13	93
	w¹¹¹⁸;; P{TRiP.GL00277 sgg RNAi}attP2/+	31	23,52	0,15	90
Positive Control	cry⁰¹	29	23,07	0,08	97
	w¹¹¹⁸	31	23,78	0,09	93

Flies (only males) were recorded in LD 12:12 for 7 days and subsequently in DD for at least 14 days. The table displays the percentage of rhythmic flies, the period length and the rhythm of all investigated genotypes in DD according to χ2-periodogram analysis. Furthermore, the number of investigated animals is indicated. Animals that died before the end of the experiment were excluded. For clarity reasons we did not included the power of the rhythmicity and the genetic background of the animals. This information can be found in Supplementary S1 Table and in S1 Fig.