Table 3. The composition (in % according to GC) of products mixture after 5 days of enzymatic oxidation of racemic decane-1,5-diol (1b).
Enzyme | OXIDATION CONDITIONS | Substrate | Products | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Coenzyme | Temperature[°C] | pH | 1b | 2b and 3b | 4b | |||||||
NAD+/ NADP+ | FMN | 25 | 35 | 45 | 7.2 | 8.5 | 9.0 | [%] | [%] | [%] | ee [%] | |
HLADH | √ | √ | √ | √ | 9 | 41 | 50 | 46 (+)-(R) | ||||
HLADH* | √ | √ | √ | √ | 23 | 36 | 41 | 30 (+)-(R) | ||||
√ | √ | √ | √ | 30 | 49 | 21 | 38 (+)-(R) | |||||
√ | √ | √ | √ | 58 | 17 | 25 | 50 (+)-(R) | |||||
√ | √ | √ | 62 | 29 | 9 | 48 (+)-(R) | ||||||
BSADH | √ | √ | √ | 68 | 29 | 3 | - | |||||
√ | √ | √ | √ | 98 | 2 | nd | ||||||
LKADH | √ | √ | √ | √ | 100 | nd | nd | |||||
√ | √ | √ | √ | 100 | nd | nd | ||||||
PADH I | √ | √ | √ | √ | 90 | 10 | nd | |||||
√ | √ | √ | √ | 100 | nd | nd | ||||||
PADH II | √ | √ | √ | √ | 83 | 9 | 8 | - | ||||
√ | √ | √ | √ | 81 | 14 | 5 | - | |||||
PADH III | √ | √ | √ | √ | 77 | 23 | nd | |||||
√ | √ | √ | √ | 68 | 32 | nd | ||||||
YADH | √ | √ | √ | √ | 100 | nd | nd | |||||
√ | √ | √ | √ | 100 | nd | nd |
*alcohol dehydrogenase recombinant from E. coli (HLADH); ee-enantiomeric excess; nd-not detected