Fig 3. Spectral binding of type I PIs with CYP3A4 in the absence (filled circles) and presence (open circles) of methamphetamine.

(A-D) The spectral binding was performed at varying concentrations of atazanavir, nelfinavir, tipranavir and lopinavir. The K_D and δA_max for each PI with and without methamphetamine are presented in Table 2. One way ANOVA with Dunnet’s post-hoc test was employed to calculate the statistical significance. A p-value <0.05 is indicated by * and <0.1 is indicated by #.