|
| ||
| Component | Amount per reaction | Final concentration |
|
| ||
| Parent vector (i.e., pTGFP-T7-Hha10T) | Variable | 600 ng/μl |
| NEB buffer 3 (10×) | 50 μl | 1× |
| Nt.BstNBI (10 U/μl) | 22.5 μl | 0.45 U/μl |
| Sterile deionized water | Variable | — |
| Total volume | 500 μl | — |
|
| ||
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