|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| Mixed plasmids (Step 20) | 8 | 24–40 ng/μl |
| NEB buffer 3 (10×) | 1 | 1× |
| SfaNI (2 U/μl) | 0.5 | 0.1 U/μl |
| SAP (0.5 U/μl) | 0.5 | 0.025 U/μl |
| Total volume | 10 | — |
|
| ||
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