|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| Denatured sample (Step 40) | 17.5 | — |
| M-MLV 5 × reaction buffer | 5 | 1× |
| dNTP (10 mM) | 1.25 | 0.5 mM |
| RNase inhibitor (40 U/μl) | 0.25 | 0.4 U/μl |
| M-MLV reverse transcriptase (200 U/μl) | 1 | 8 U/μl |
| Total volume | 25 | — |
|
| ||
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