|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| PCR template (Step 41) | 2 | — |
| Phusion HF buffer (5×) | 20 | 1× |
| dNTP (10 mM) | 2 | 0.2 mM |
| Forward primer (10 μM) | 2 | 0.2 μM |
| Reverse primer (10 μM) | 2 | 0.2 μM |
| Phusion DNA polymerase (2 U/μl) | 0.4 | 0.008 U/μl |
| Nuclease-free water | 71.6 | — |
| Total volume | 100 | — |
|
| ||
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