|
| |||
| Cycle number | Denaturation | Annealing | Extension |
|
| |||
| 1 | 98 °C, 30 s | — | — |
| 2–36 | 98 °C, 10 s | 60 °C, 30 s | 72 °C, 15 s |
| 37 | — | — | 72 °C, 5 min |
|
| |||
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