|
| ||
| Component | Amount per reaction | Final concentration |
|
| ||
| Purified PCR product (Step 45) | Variable | 10–20 ng/μl |
| NEB buffer 3 (10×) | 1 μl | 1× |
| NcoI (10 U/μl) | 0.5 μl | 0.5 U/μl |
| SAP (1 U/μl) | 0.5 μl | 0.05 U/μl |
| Sterile deionized water | Variable | — |
| Total volume | 10 μl | — |
|
| ||
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