Skip to main content
. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Nat Protoc. 2015 Aug 20;10(9):1389–1406. doi: 10.1038/nprot.2015.094
Component Amount per reaction Final concentration
T4 PNK buffer (10×) 2 μl 1×
T4 PNK (10 U/μl) 0.5 μl 0.25 U/μl
ATP (10 mM) 2 μl 1 mM
12-mer ODN Variable 10 μM
13-mer ODN (100 μM) 2 μl 10 μM
Sterile deionized water Variable —
Total volume 20 μl —