|
| ||
| Component | Amount per reaction (μ l) | Final concentration |
|
| ||
| Reaction mixture (Step 46A(ii)) | 15 | — |
| NEB buffer 3 (10×) | 0.5 | 1× |
| SfaNI (2 U/μl) | 1 | 0.1 U/μl |
| Sterile deionized water | 3.5 | — |
| Total volume | 20 | — |
|
| ||
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