|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| Reaction mixture (Step 46B(i)) | 10 | — |
| CutSmart buffer (10×) | 0.5 | 1× |
| T4 PNK (10 U/μl) | 0.5 | 0.33 U/μl |
| [γ-32P]ATP (5 μCi/μl) 10 | 0.5 | 0.17 μCi/μl |
| ATP (100 μM) | 0.1 | 0.67 μM |
| Sterile deionized water | 3.4 | — |
| Total volume | 15 | — |
|
| ||
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