|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| Reaction mixture (Step 46C(i)) | 150 | — |
| NEB buffer 3 (10×) | 5 | 1× |
| NcoI (10 U/μl) | 5 | 0.25 U/μl |
| Sterile deionized water | 40 | — |
| Total volume | 200 | — |
|
| ||
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