|
| ||
| Component | Amount per reaction | Final concentration |
|
| ||
| Phosphorylated ODNs (Step 11) | 15 μl | ~1.25 μM |
| Gapped plasmid (Step 10) | Variable | ~0.125 μM |
| Ligation buffer (10×) | 12 μl | 1× |
| Sterile deionized water | Variable | — |
| Total volume | 120 μl | — |
|
| ||
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