|
| ||
| Component | Amount per reaction (μl) | Final concentration |
|
| ||
| Annealed product (Step 16) | 120 | — |
| Ligation buffer (10×) | 8 | 1× |
| ATP (10 mM) | 20 | 1 mM |
| T4 DNA ligase (400 U/μl) | 20 | 40 U/μl |
| Sterile deionized water | 32 | — |
| Total volume | 200 | — |
|
| ||
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